Mitigation of carbon dioxide emissions with microalgae has recently been one of the hottest topics in carbon capture, sequestration, and utilization (CCS&U). Photoautotrophic cultivation of microalgae is considered the most promising platform for CO₂ capture and re-utilization as the microalgal biomass obtained from bio-fixation of CO₂ can be further used as feedstock/raw materials for biofuel and bio-based chemicals production. To facilitate the commercialization of microalgae-based industry, our team has developed platform technologies to improve and integrate the currently available microalgae biorefinery tools, making them more economically feasible for practical applications. The major research directions include (1) creating microalgae mutants with the enhancement in their growth rate, temperature tolerance and carbon dioxide fixation efficiency; (2) developing large-scale outdoor microalgae cultivation systems using industrial flue gas as carbon source and employing optimal operation strategies to further improve the CO₂ fixation efficiency; (3) establishing effective methods for the production of microalgae-based biofuels and high-value products; the residual algal cells or byproducts (e.g., glycerol) are used to produce value-added products (e.g., pigments, DHA/EPA, aquacultural feeds, cosmetic products, etc.) to enhance the economic feasibility of the overall process; (4) establishing effective tools for cell disruption and extraction of functional components from microalgae for follow-up downstream processing; and (5) Utilizing wastewater to capture CO₂ via alkaline absorption and cultivate microalgae using the CO₂-loaded wastewaters.